Publications

Publications

Bilodeau, ML and HE Hamm. Regulation of PAR1 and PAR4 signaling in human platelets by compartmentalized cyclic nucleotide actions J. Pharmacol. Exp. Ther. 2007 May 24 [Epub ahead of print]]

Oldham, WM, N Van Eps, AM Preininger, WL Hubbell, HE Hamm. Mapping allosteric connections from the receptor to the nucleotide-binding pocket of heterotrimeric G proteins. Proc Natl Acad Sci USA 104(19): 7927-7932, 2007.]

Voss, Bryan, JN McLaughlin, M Holinstat, R Zent, HE Hamm. PAR1, but not PAR4, activates human platelets through a Gi/o/PI3K signaling axis. Mol. Pharmacol. 71(5): 1399-1406, 2007.]

Huang, Kui, B Voss, D Kumar, HE Hamm, Eva Harth. Dendritic Molecular Transporters Provide Control of Delivery to Intracellular Compartments. Bioconjugate Chem. 18(2):403-9, 2007.]

Spiegelberg, BD, HE Hamm. Roles of G-protein-coupled receptor signaling in cancer biology and gene transcription. Curr. Opin. Genet. Dev. Feb;17(1):40-4, 2007.]

Murphy, SC, T Harrison, HE Hamm, JW Lomasney, N Mohandas, K Haldar. Erythrocyte G Protein as a Novel Target for Malarial Chemotherapy. PLoS Med. 3(12): 2403-15, 2006.]

Holinstat, M, B Voss, ML Bilodeau, HE Hamm. Protease activated receptors differentially regulate human platelet activation through a phosphatidic acid-dependent pathway. Mol. Pharmacol. [Epub ahead of print, Dec. 6, 2006.]

Van Eps N, WM Oldham, HE Hamm, WL Hubbell. Structural and dynamical changes in an a-subunit of a heterotrimeric G protein along the activation pathway. Proc Natl Acad Sci USA. 103(44):16194-9, 2006.

Chen, S and HE Hamm. DEP Domains: More Than Just Membrane Anchors. Dev Cell 11(4):436-8, 2006.

Oldham, WM and HE Hamm. Structural Basis of Function in Heterotrimeric G Proteins. Quarterly Reviews of Biophysics 39(2):117-166, 2006.

Holinstat, M, WM Oldham, HE Hamm. G-protein-coupled receptors: evolving views on physiological signaling. EMBO Reports 7(9):866-869, 2006.

Oldham, W, N Van Eps, A Preininger, W Hubbell, HE Hamm. Mechanism of the receptor-catalyzed activation of heterotrimeric G proteins. Nature Structural and Molecular Biology, 13(9):772-7, 2006.

Holinstat, Michael, Bryan Voss, Matthew Bilodeau, Joseph McLaughlin, John Cleator, and Heidi E. Hamm. PAR4, but not PAR1, signals human platelet aggregation via Ca2+ mobilization and synergistic P2Y12 receptor activation. J. Biol. Chem. [Epub ahead of print, July 12, 2006].

Ding J., Jaime N. Guzman, Tatiana Tkatch, Songhai Chen, Joshua A. Goldberg, Philip J. Ebert, Pat Levitt, Charles J. Wilson, Heidi E. Hamm and D. James Surmeier. RGS4-dependent attenuation of M(4) autoreceptor function in striatal cholinergic interneurons following dopamine depletion. Nature Neuroscience Jun:9(6):832-834, 2006.

Caruso G., P. Bisegna, L. Shen, D. Andreucci, Heidi Hamm, and E. Dibenedetto. Modeling the Role of Incisures in Vertebrate Phototransduction. Biophys. J. 91(4):1192-212, 2006.

Preininger A.M., L.G. Henage, W.M. Oldham, E.J. Yoon, Heidi Hamm , and H.A. Brown. Direct modulation of phospholipase D activity by G bg . Mol. Pharmacol . 2006 Apr 25; [Epub ahead of print].

Photowala H., T. Blackmer, E. Schwartz, Heidi Hamm , and Simon Alford. G protein betagamma-subunits activated by serotonin mediate presynaptic inhibition by regulating vesicle fusion properties. PNAS , 103(11):4281-6, 2006.

Bilodeau, M.L. and Heidi Hamm. eNOS reveals a new face in G protein signaling. Mol Pharmacol. 2005 Dec 23

Spiegelberg, Bryan D. and Heidi E. Hamm.Gbg binds HDAC5 and inhibits its transcriptional co-repression activity.  J. Biol. Chem., 280:41769, 2005.

Cleator, John H., Wen Qin Zhu, Douglas E. Vaughan, and Heidi E. Hamm.  Differential regulation of endothelial exocytosis of P-selectin and von Willebrand Factor by protease-activated receptors and cAMP.  Blood, [Epub ahead of print, Dec. 6] in press, 2005.

Georgoussi, Zafiroula, Leonidas Leontiadis, Georgia Mazarakou, Manolis Merkouris, Karren Hyde, and Heidi Hamm.  Selective interactions between G protein subunits and RGS4 with the C-terminal domains of the μ- and δ-opioid receptors regulate opioid receptor signaling.  Cellular Signaling, [Epub ahead of print, Aug. 22] in press, 2005.

Caruso, G., H. Khanal, V. Alexiades, F. Rieke, H.E. Hamm and E Dibenedetto.  Mathematical and computational modeling of spatio-temporal signaling in rod phototransduction. IEE Systems Biology, in press, 2005.

Chen, Songhai, Fang Lin, Heidi Hamm.  RACK1 binds to a signal transfer region of Gbγ and inhibits PLCb2 activation.  J. Biol. Chem., 280:33445, 2005.

McLaughlin, Joseph N., Lixin Shen, Michael Holinstat, Joshua D. Brooks, Heidi Hamm.  Functional selectivity of G protein signaling by agonist peptides and thrombin for the protease-activated receptor-1.  J. Biol. Chem., 280:25048, 2005.

Lin, F., D.S. Sepich, S. Chen, J. Topczewski, Chunyeu Yin, L. Solnica-Krezel and H.E. Hamm. Essential roles of Gα 12/13 signaling in distinct cell behaviors driving zebrafish convergence and extension gastrulation movements.  J. Cell Biol., 169(5):777-87, 2005.

Lukov, George L., Ting Hu, Joseph N. McLaughlin, Heidi E. Hamm, Barry M. Willardson.  Phosducin-like protein acts as a molecular chaperone for G protein bγ dimer assembly.  EMBO J., 24(11):1965-1975, 2005.

Gerachshenko, Tatyana, Trillium Blackmer, Eun-Ja Yoon, Cheryl Bartleson, Heidi E. Hamm and Simon Alford.  Gβγ acts at the C-terminus of SNAP-25 to mediate presynaptic inhibition.  Nature Neuroscience 8(5):597-605, 2005.

McLaughlin, J.N., M.R. Mazzoni, J.H. Cleator, L. Earls, A.L. Perdigoto, J.D. Brooks, J.A. Muldowney III, D.E.  Vaughan, H.E. Hamm. Thrombin modulates the expression of a set of genes including thrombospondin-1 in human micro-vascular endothelial cells.  J. Biol. Chem., 280:22172-22180, 2005.

Blackmer, T., E.C. Larsen, C. ,C. Bartleson, J.A. Kowalchyk, E-J Yoon, A.M. Preininger, S. Alford, H.E. Hamm and T.F.J. Martin. G protein βγ directly regulates the SNARE protein fusion machinery for secretory granule exocytosis. Nature Neuroscience, 8(4):421-425, 2005.

Cabrera-Vera TM, S. Hernandez, L.R. Earls, M. Medkova, A.K. Sundgren-Andersson, D.J. Surmeier, and H.E. Hamm.  RGS9-2 modulates D2 dopamine receptor-mediated Ca2+ channel inhibition in rat striatal cholinergic interneurons.
Proc. Natl. Acad. Sci. USA 101(46):16339-44, 2004.

Srinivasan, C., J. Toon, L. Amari, A.M. Abukhdeir, H.E. Hamm, C.F.G.C. Geraldes, Y-K Ho, D.M. de Freitas. Competition between Lithium and Magnesium Ions for the G-Protein Transducin in the Guanosine 5’-Diphosphate Bound Conformation. J. Inorganic Biochem. 98:691-701, 2004.

Chen, Songhai, Bryan D. Spiegelberg, Fang Lin, Edward J. Dell, Heidi E. Hamm.  Interaction of Gbg with RACK1 and other WD40 repeat proteins.  J. Molecular & Cellular Cardiology 37(2):399-406, 2004.

Chen, S. E.J. Dell, F. Lin, J. Sai, H.E. Hamm. RACK1 regulates specific functions of Gbg.  J. Biol. Chem. 279(17):17861-8, 2004.

Preininger, A.M., H.E. Hamm. G-protein signaling: Insights from new structures. Sci. STKE Jan. 27 (218), 2004.

Harrison, T., B. Samuel, T. Akompong, H. Hamm, N. Mohandas, J. Lomasney, K. Haldar. Erythrocyte G protein signaling as a target for malaria infection. Science 301:1734-6, 2003.

Cabrera-Vera, T.M., J. Vanhauwe, T.O. Thomas, M. Medkova, A. Preininger, M. Mazzoni and H.E. Hamm, Insights into G protein structure, function and regulation.  Endocrine Reviews 24:765-781, 2003.

Slessareva JE, K.M. Depree, H. Ma, T.M. Cabrera-Vera, L.A. Flood, H.E. Hamm and S.G. Graber. Closely related G protein-coupled receptors use multiple and distinct domains on G protein α subunits for selective coupling.   J. Biol.  Chem. 278:50530-50536, 2003.

Andreucci, D., P. Bisegna, E. DiBenedetto, H.E. Hamm. Mathematical Model of the Spatio-temporal Dynamics of Second Messengers in Visual Transduction. Biophysical Journal 85 (3):1358-1376, 2003.

Preininger, A., N. VanEps, N-J Yu, M. Medkova, W. Hubbell, and H.E. Hamm. The myristoylated amino terminus of Gail plays a critical role in the structure and function of Gail subunits in solution. Biochemistry 42:7931-7941, 2003.

Chen, N.-F., J.-Z. Yu, N.P. Skiba, H.E. Hamm, and M.M. Rasenick.  A specific domain of Gia required for the transactivation of Gia by tubulin is implicated in the organization of cellular microtubules.  J. Biol. Chem. 278:15285-90, 2003. 

Dell, E.J., J. Conner, E.G. Stebbins, N.P. Skiba, D. Mochly-Rosen, H.E. Hamm. The βγ subunit of heterotrimeric G proteins interacts with RACK1 and two other WD repeat proteins. J Biol Chem. 277:49888-95, 2002.

Vanhauwe J.F., T.O. Thomas, R.D. Minshall, C. Tiruppathi, A. Li, A. Gilchrist, E.-J. Yoon, A.B. Malik, and H.E. Hamm. Thrombin receptors activate Go proteins in endothelial cells to regulate intracellular calcium and cell shape changes. J Biol Chem. 277:34143-9, 2002.

Medkova, M, N.-J. Yu, A.M. Preininger, W.L. Hubbell, and H.E. Hamm, Conformational changes in the amino terminal helix of the G protein α subunit following dissociation from Gbg subunit and activation.  Biochemistry 41:9962-72, 2002.

Mukherjee, S., V. Gurevich, A. Preininger, H.E. Hamm, M.F. Bader, A.T. Fazleabas, L. Birnbaumer and M. Hunzicker-Dunn. Aspartic acid 564 in the third cytoplasmic loop of the luteinizing hormone/choriogonadotropin receptor is crucial for phosphorylation-independent interaction with arrestin2.  J. Biol. Chem. 227:17916-17927, 2002.

Dell, E., T. Blackmer, N.P. Skiba, Y. Daaka, L.R. Shekter, R. Roals, E. Reuveny, and H.E. Hamm.  Defining G protein βγ specificity for effector recognition. Meth. Enzymol. 344:421-434, 2002.

Cabrera-Vera T.M., T.O. Thomas, J. Vanhauwe, K.M. Depree, S.G. Graber, H.E. Hamm.  Dissecting receptor-G protein specificity using Gα chimeras. Meth. Enzymol. 344:69-81, 2002.

Gilchrist A., A. Li, H.E. Hamm.  Design and use of C-terminal minigene vectors for studying role of heterotrimeric G proteins.  Methods Enzymol. 344:58-69, 2002.

Schey, K.L., M. Busman, L.A. Cook, N. Skiba, H.E. Hamm and J.D. Hildebrandt, Structural Characterization of Intact G-Protein γ-Subunits by Mass Spectrometry. Meth. Enzymol. 344:586-597, 2002.

Hamm H.E.  How activated receptors couple to G proteins. Proc.  Natl. Acad. Sci.  U.S.A.  98:4819-4821, 2001.

Gilchrist A, J.F. Vanhauwe, A. Li, T.O. Thomas, T. Voyno-Yasenetskaya and H.E. Hamm. Gα minigenes expressing C-terminal peptides serve as specific inhibitors of thrombin-mediated endothelial activation. J Biol Chem. 276:25672-9, 2001.

Thulin C.D, J.R. Savage, J.N. McLaughlin, S.M. Truscott, W.M. Oldham, N.G. Ahn, K.A. Resing, H.E. Hamm, M.W. Bitensky and B.M. Willardson. Modulation of the G-protein regulator phosducin by Ca2+ /calmodulin-dependent protein kinase II phosphorylation and 14-3-3 protein binding.  J Biol Chem 276:23805-15, 2001.

Blackmer T., E.C. Larsen, M. Takahashi, T.F. Martin, S. Alford and H.E. Hamm. G protein βγ subunit-mediated presynaptic inhibition: regulation of exocytotic fusion downstream of Ca2+ entry.  Science 292:293-297, 2001.

Minadeo, N., B. Layden, L.V. Amari, V. Thomas, K. Radloff, C. Srinivasan, H.E. Hamm and D. Mota de Freitas  Effect of Li+ upon the Mg2+-dependent activation of recombinant Giα1.  Arch. Biochem. Biophys. 388:7-12, 2001.

Thomas, T.O., H. Bae, M. Medkova and H.E. Hamm, An intramolecular contact in Gα transducin that participates in maintaining its intrinsic GDP release rate.  Mol Cell Biol Res Commun 4:282-91, 2001.

Lopez, I., E.C. Mak, J. Ding, H.E. Hamm and J.W. Lomasney.  A novel bifunctional phospholipase c that is regulated by Gα12 and stimulates the Ras/MAP kinase pathway. J. Biol. Chem. 276:2758-2765, 2001.

Aris, L., A. Gilchrist, S. Rens-Domiano, C. Meyer, P. Schatz, E. Dratz and H.E. Hamm. Structural Requirements for the Stabilization of Metarhodopsin II by the C terminus of the α subunit of transducin.  J. Biol. Chem. 276:2333-2339, 2001.

Hernandez-Lopez, S., T. Tkatch, E. Perez-Garci, E. Galarraga, J. Bargas, H. Hamm, and D. J. Surmeier.  D2 dopamine receptors in striatal medium spiny neurons reduce L-type Ca2+ currents and excitability through a novel PLCβ1/IP3/calcineurin signaling cascade.  J. Neurosci. 20:8987-8995, 2000.

Savage, J.R., J.N. McLaughlin, N.P. Skiba, H.E. Hamm, and B.M. Willardson. Functional roles of the two domains of phosducin and phosducin-like protein. J. Biol. Chem. 275:30399-407, 2000.

Minshall, R.D., C. Tiruppathi, S. Vogel, W.D. Niles, A. Gilchrist, H.E. Hamm and A.B. Malik.  Endothelial cell surface gp60 activates vesicle formation via Gi-coupled src kinase signaling pathway.  J. Cell Biol. 150:1057- 1070, 2000.

Mazzoni, M.R, S. Taddei, L. Giusti, P. Rovero, C. Galoppini, A. D’Ursi, S. Albrizio, Triolo, E. Novellino, G. Greco, A. Lucacchini and H.E. Hamm.  A Gαs carboxyl-terminal peptide prevents Gs activation by the A2A adenosine receptor Mol. Pharm. 58:226-236, 2000.

Skiba, N.P., T.O. Thomas, and H. E. Hamm.  Gat/Gai1 chimeras used to define the structural basis of specific functions of Ga.  Meth. Enzymol. 315:502-523, 2000.

Yang, C.S., N. P. Skiba, M.R. Mazzoni, T.O. Thomas and H.E. Hamm. Fluorescent probes as indicators of conformation changes in transducin on activation.  Meth. Enzymol. 315:490-501, 2000.

Mazzoni, M.R. and H.E. Hamm, Limited proteolytic digestion studies of G protein-receptor interactions. Meth. Enzymol. 315:363-376, 2000.

Gilchrist, A., A. Li and H.E. Hamm.  Use of peptides-on-plasmids combinatorial library to identify high affinity peptides that bind rhodopsin.  Meth. Enzymol. 315:388-403, 2000.

Ellis, C.A., A.B. Malik, A. Gilchrist, H. Hamm, R. Sandoval, T. Voyno Yasenetskaya, and C. Tiruppathi. Thrombin induces PAR-1 gene expression in endothelial cells via activation of Gi-linked Ras/MAPK Pathway. J. Biol. Chem. 274:13718-13727, 1999. 

Bae, H., T.M. Cabrera-Vera, K.M. Depree, S.G. Graber and H.E. Hamm. Two amino acids within the α4 helix of Gαi1 mediate coupling with 5-hydroxytryptamine1B receptors. J. Biol. Chem. 274:14963-14971, 1999.

Rajagopalan-Gupta, R.M., S. Mukherjee, X. Zhu, Y.K. Ho, H.E. Hamm, M. Birnbaumer, L. Birnbaumer and M. Hunzicker-Dunn.  Roles of Gi and Gq/11 in mediating desensitization of the luteinizing hormone/choriogonadotropin receptor in porcine ovarian follicular membranes.  Endocrinology 140:1612-1621, 1999.

Skiba, N.P., C.S. Yang, T. Huang, H. Bae and H.E. Hamm. The α-helical domain of Gαt determines specific interaction with RGS9.   J. Biol. Chem. 274:8770-8778, 1999.

Gilchrist, A., M. Bunemann, A. Li, M.M. Hosey and H.E. Hamm. A dominant negative strategy for studying roles of G proteins in vivo. J. Biol. Chem. 274:6610-6616, 1999.

Yang, C.S., N. Skiba, M. Mazzoni and H.E. Hamm. Conformational changes at the carboxyl-terminus of Gtα occur during G protein activation. J.Biol. Chem. 274:2379-2385, 1999. 

Gilchrist, A., M. Mazzoni, B. Dineen, A. Dice, J. Linden, W.R. Proctor, C.R. Lupica, T. Dunwiddie, and H.E. Hamm.  Antagonists of the receptor -G protein interface block Gi-coupled signal transduction. J. Biol. Chem. 273:14912-12919, 1998.

Skiba, N.P. and H.E. Hamm.  How Gsα activates adenylyl cyclase. Nature Structural Biol. 5:85, 1998. 

Hamm, H. E.  The many faces of G protein signaling.  J. Biol. Chem.  273:669-672, 1998.

Ford, C.E., N. Skiba, H. Bae, Y. Daaka, E. Reuveny, L. Shekter, R. Rosal, G. Weng, C.S. Yang, R. Iyengar, R. Miller, L.Y. Jan, R.J. Lefkowitz and H.E. Hamm. Molecular basis for interactions of G protein βγ subunits with effectors. Science 280:1271-1274, 1998.

Dratz, E.A., J.E. Furstenau, C.G. Lambert, D.L. Thireault, H.M. Rarick, T. Schepers, S. Pakhlevaniants and H.E. Hamm.  Correction. NMR structure of a receptor-bound G protein peptide.  Nature 390:424, 1997.

Bae, H., K. Anderson, L.A. Flood, N.P. Skiba, H.E. Hamm and S.G. Graber. Molecular determinants of selectivity in 5HT1 receptor Gi interactions.  J. Biol. Chem. 272:32071-32077, 1997.

Nekrasova, E.R., D.M. Berman, R.R. Rustandi, H.E. Hamm, A.G. Gilman and V.Y. Arshavsky.  Activation of transducin GTPase by two proteins of the RGS family.  Biochemistry 36:7638-7643, 1997.

Mazzoni, M.R., N.O. Artemyev and H.E. Hamm.  Proteolytic fragmentation for epitope mapping.  Methods in Molecular Biology 66:109-120, 1996.

Mazzoni, M.R. and H.E. Hamm.  Interaction of transducin with light-activated rhodopsin protects it from proteolytic digestion by trypsin.  J. Biol. Chem. 271:30034-30040, 1996.

Artemyev, N.O., R. Surendrau, J.C. Lee, and H.E. Hamm.  Subunit structure of rod cGMP phosphodiesterase.  J. Biol. Chem. 271:25382-25388, 1996.

Artemyev, N.O., M. Natochin, M. Busman, K.L. Schey and H.E. Hamm.  Mechanism of photoreceptor cGMP PDE inhibition by its γ subunits.  Proc. Natl. Acad. Sci. 93:5407-5412, 1996.

Hamm H.E. and A. Gilchrist. Heterotrimeric G proteins.  Curr. Opinion in Cell Biol. 8:189-196, 1996.

Skiba, N.P., H. Bae and H.E. Hamm.  Mapping of effector binding sites of transducin α subunit using Gαt/Gαi chimeras.  J. Biol. Chem. 271:413-424, 1996.

Martin, E.L., S. Rens--Domiano, P.J. Schatz and H.E. Hamm.  Potent peptide analogues of a G protein receptor-binding region obtained with a combinatorial library.  J. Biol. Chem. 271:361-367, 1996.

Lambright, D.G., J. Sondek, A. Bohm, N.P. Skiba, H.E. Hamm and P.B. Sigler.  The 2.0Åcrystal structure of a heterotrimeric G protein.  Nature 379:311-319, 1996.

Sondek, J., D.G. Lambright, A. Bohm, H.E. Hamm and P.B. Sigler.  Crystal structure of a G-protein beta gamma dimer at 2.1A resolution.  Nature 379:369-374, 1996.
a. Accompanied by a News and Views article.  The G-protein nanomachine.  D. E. Clapham.  Nature 379:297-299

Rens-Domiano, S. and H.E. Hamm.  Structural and functional relationships of heterotrimeric G proteins.  FASEB J. 9:1059-1066, 1995.

Suh, K.H. and H.E. Hamm. Cyclic AMP-dependent phosphoprotein components I and II interact with βγ subunits of transducin in frog rod outer segments.  Biochem. 35:290-298, 1995.

Slepak, V.Z., N.O. Artemyev, Y. Zhu, C.L. Dumke, L. Sabacan, J. Sondek, H.E. Hamm, M. D. Bownds and V. Y. Arshavsky.  An effector site that stimulates G protein GTPase in photoreceptors.  J. Biol. Chem. 270:14319-14324, 1995.

Skiba, N.P., N.O. Artemyev and H.E. Hamm.  The carboxyl terminus of the β subunit of rod cGMP phosphodiesterase contains distinct sites of interaction with the enzyme catalytic subunits and the α subunit of transducin.  J. Biol. Chem. 270:13210-13215, 1995.